Figure 3: The effect of I281T mutation on interactions with CAR partners. Activation of the mammalian two-hybrid GAL4 reporter by wild-type CAR (black columns) and I281T mutant (grey columns) co-transfected with fusion proteins of either NR coactivator SRC1, NR corepressor NCoR or the heterodimer partner RXRα and after exposure to either vehicle DMSO (D), 1 μM CITCO (C) or 10 μM S07662 (S). The results were normalized to the activity of wild-type CAR in the presence of DMSO (set at 100). The data shown are means ± SEM of six biological replicates. The symbols * and # indicate a statistically significant difference (p < 0.017) to wild-type CAR or to the relevant DMSO control, respectively.