Table 3: Binding affinities of LXRα mutants for PPARα in the absence or presence of ligands.


Wild-type K d (nM) H383E K d (nM) E387Q K d (nM) H390E K d (nM) L414R K d (nM) R415A K d (nM)

No ligand 8 ± 2 7 ± 1 10 ± 2 27 ± 11 13 ± 5 14 ± 3
T0901317 35 ± 6 13 ± 2 53 ± 9 9 ± 1 34 ± 6 23 ± 17
25HC 16 ± 3 2 ± 0.5 49 ± 12 5 ± 1 2 ± 0.7 67 ± 13
C16:0 FA 104 ± 40 7 ± 1 27 ± 11 8 ± 1 2 ± 0.2 39 ± 22