|Figure 8: Effect of LXRα interface mutations on luciferase reporter activation of human ApoA1
promoter. COS-7 cells were co-transfected with pSG5 empty vector or each indicated
LXRα plasmid and transactivation of the ApoA1 luciferase reporter construct in the
presence of vehicle (solid bars) and 25-HC (Gray bars) was measured. Luciferase reporter
activity was measured 18 hrs after the addition of vehicle or ligand and normalized
using Renilla as an internal control. Asterisks denote significant differences due
to the single point mutations compared to wild-type LXRα for vehicle or 25-HC treated
cells: *p<0.05, **p<0.01, ***p<0.001.