Figure 7: Effect of LXRα interface mutations on luciferase reporter activation of human SREBP-1c promoter. COS-7 cells were co-transfected with pSG5 empty vector or each indicated LXRα plasmid and transactivation of the SREBP-1c LXRE-luciferase reporter construct in the presence of vehicle (solid bars) and 25-HC (Gray bars) was measured. Luciferase reporter activity was measured 18 hrs after the addition of vehicle or ligand and normalized using Renilla as an internal control. Asterisks denote significant differences due to the single point mutations compared to wild-type LXRα for vehicle or 25-HC treated cells: *p<0.05, **p<0.01, ***p<0.001.