|Figure 7: Effect of LXRα interface mutations on luciferase reporter activation of human SREBP-1c
promoter. COS-7 cells were co-transfected with pSG5 empty vector or each indicated
LXRα plasmid and transactivation of the SREBP-1c LXRE-luciferase reporter construct
in the presence of vehicle (solid bars) and 25-HC (Gray bars) was measured. Luciferase
reporter activity was measured 18 hrs after the addition of vehicle or ligand and
normalized using Renilla as an internal control. Asterisks denote significant differences
due to the single point mutations compared to wild-type LXRα for vehicle or 25-HC
treated cells: *p<0.05, **p<0.01, ***p<0.001.