|Figure 5: Far UV CD of the mixture of PPARα and LXRα proteins. Experimentally observed (Obs,
open circles) circular dichroic spectrum of a mixture of 0.2 µM PPARα and 0.2 µM (A)
wild-type, (B) H383E, (C) E387Q, (D) H390E, (E) L414R, and ((F) R415A LXRα compared
to the calculated average (Calc, closed circles) of the individually obtained PPARα
and LXRα spectra representing non-interacting proteins. The amino acid molarity for
each spectrum was 0.0002 M, and each spectrum represents the average of at least three
replicates, scanned 5 times per replicate.