Figure 5: Far UV CD of the mixture of PPARα and LXRα proteins. Experimentally observed (Obs, open circles) circular dichroic spectrum of a mixture of 0.2 µM PPARα and 0.2 µM (A) wild-type, (B) H383E, (C) E387Q, (D) H390E, (E) L414R, and ((F) R415A LXRα compared to the calculated average (Calc, closed circles) of the individually obtained PPARα and LXRα spectra representing non-interacting proteins. The amino acid molarity for each spectrum was 0.0002 M, and each spectrum represents the average of at least three replicates, scanned 5 times per replicate.