Binding Site Analysis of the

Table 3: Average brood size following RNAi of selected genes.


Stage	Gene	N2 (WT)	eri-1(-)	jun-1 (-)	vab-2 (-)

	GFP	51.3 ± 9.6	44.8± 4.4	37.5± 6.4	40.3± 5.7
co-located	hmg-1.2	47.6± 8.7	1.9± 0.6*	0	34.4± 4.8*
co-located	dcp-66	53.7± 8.4	8.3± 0.8**	2.1 ± 0.9*	14.9 ± 1.1
co-located	puf-9	48.3± 9.1	37.8±3.9	9.7 ± 3.7*	21.7 ± 4.1*
co-located	pas-5	0	0	0	0
co-located	let-526	47.8± 5.6	0	0.7 ± 0.3	36.6± 2.2
co-located	hpl-2	51.3± 7.3	41.7± 6.1	38.1± 6.6	44.3± 3.6
co-located	sop-3	49.6± 8.4	44.6± 4.3	35.9± 5.2	39± 4.2
co-located	sor-1	0	0	0	0
co-located	bet-1	44.3± 6.6	2.6± 1.3***	4.6 ± 1.5**	13 ± 2.8*
co-located	gei-1	49.9± 11.3	0.7± 0.3	14.3 ± 3.5**	38.9± 5.1
co-located	gei-13	5.4± 1.2*	0.7± 0.5	6.1± 1.6	19.3± 3.2
late L3/early L4	gex-3	52.6±7.8	10.9±2.3**	13 ±2.1	17.8 ± 2.9**
mid L4	lin-31	55.9± 5.3	38.6± 3.6	10.6 ±1.3**	39.3±3.7

Average brood size ± SD. N = 15 for each experiment. * = presence of abnormal eggs at ≥ 10% average; = presence of abnormal eggs at ≥ 25% average; * = presence of abnormal eggs at ≥ 50% average. Bolded data indicate synergistic enhancement of brood size phenotype in jun-1 and vab-2 mutants compared to N2.