Figure 1: NHR-6::eGFP ChIP. A. Fluorescent and DIC images of NHR-6::eGFP localization in the spermathecae of late L3 (sgIs1) and mid L4 animals (sgIs2). White brackets indicate location of spermatheca cells. The surrounding punctate signal is gut autofluorescence, not GFP. B. Spermatheca precursor cell (SPC) lineage during L3 and L4. Green bars indicate time points at which animals were fixed for ChIP. C. Immunoblot of eGFP immunoprecipated from mixed stage sgIs1 (Transgenic) and wildtype (WT) animals with immobilized polyclonal antibody.