Figure 2: Renin and ACE/AT1r protein and gene expression in the kidney. The high- fructose administration leads to an increase in protein and gene expression of Renin, ACE and AT1r and GW501516 had no effects on these parameters (n=5/group). A: mRNA levels of renin, ACE, and AT1r. B: RENIN protein expression. C: ACE protein expression. D: AT1r protein expression. Data are presented as mean ± SD. **, *** and **** indicate P<0.01, P<0.001 and P<0.0001, respectively, compared with the SC group; †† indicates P<0.01 compared with the SC+GW group.