Figure 1: A detail referring to the pathways downstream of the TGF-β growth factor depicted in the Molecular Interaction Map (MIM) depicted in [12] (reproduced with permission of 2008-2015 Impact Journals, LLC). The text explanation is slightly modified from the Supplementary Material - Annotation List of [12]. The symbol # followed by an Arabic number indicates an interaction or a contingency depicted in the MIM. Syntactic rules of the MIM are depicted in Fig. 2. Dimeric TGF-β (#108 – number in the annotation list of [12] binds with high affinity (#110) type II (TGFβR-II) receptors dimers (#109), on the cell surface. Dimeric TGF-β type I receptor (TGFβR-I) (#111) binds the dimeric [TGFβR-II: dimeric TGFβ complex] (#112). In the hetero-tetrameric receptor complex, type II receptors phosphorylate a serine/threonine-rich region (the GS region), located in the kinase domain of TGFβR-I (#113), which then propagates the signal. An activated TGF-β complex can bind (#127) and phosphorylate Smad3 and Smad2 (#129, #130). Smad4 can bind activated Smad2 and Smad3 (#132) forming complexes that can translocate into the nucleus (#133). These SMAD complexes can then bind to a SMAD binding site [TFBS:SMAD] on MYC, repressing its transcription. SMAD4 (most likely complexed with phosphorylated SMAD2 or SMAD3) also binds (an additional example) to the promoter region of CCND1 upon a TGF-β stimulus, repressing transcription.