Figure 2: PXR mRNA expression increases in intestinal epithelial cells and murine enterocytes upon differentiation. Real-time qPCR analysis of PXR expression in undifferentiated Caco-2 cells treated with the differentiating agent, sodium butyrate (1 μM). Undifferentiated Caco-2 cells were allowed to differentiate for 7-days post-confluence, in the presence of sodium butyrate. On analysis we observed that PXR mRNA levels increased several fold upon sodium butyrate-induced differentiation. Gene expression changes were calculated using the comparative Ct method with β-actin and GAPDH as the reference genes. Data expressed as fold change in mRNA expression compared to undifferentiated cells. Experiment was performed at least two times in triplicate. Graph show mean values ± s.e.m. *P ≤ 0.05 (One-way ANOVA) [212].