|Figure 4: Examples of immunohistochemical staining illustrate the effect of cuprizone and NR
agonists on microglia cells. Shown are sections of Cc following treatments; (a), (b)
control condition, normal chow; (c), (d) after three weeks of cuprizone feeding; (e),
(f) combined cuprizone feeding with injection of all-trans RA; (g), (h) combined treatment
of cuprizone with 9-cis RA; and (i), (j) combined treatment of cuprizone with T0901317.
Panels (a), (c), (e), (g), and (i) represent areas in the caudal, panels (b), (d),
(f), (h), and (j) in the rostral Cc. Microglia cells were marked with an antiserum
against ionized calcium binding adapter molecule-1 (Iba1). Scale bar 50 µm in panel
j is valid for all photographs.