Figure 4: Examples of immunohistochemical staining illustrate the effect of cuprizone and NR agonists on microglia cells. Shown are sections of Cc following treatments; (a), (b) control condition, normal chow; (c), (d) after three weeks of cuprizone feeding; (e), (f) combined cuprizone feeding with injection of all-trans RA; (g), (h) combined treatment of cuprizone with 9-cis RA; and (i), (j) combined treatment of cuprizone with T0901317. Panels (a), (c), (e), (g), and (i) represent areas in the caudal, panels (b), (d), (f), (h), and (j) in the rostral Cc. Microglia cells were marked with an antiserum against ionized calcium binding adapter molecule-1 (Iba1). Scale bar 50 ┬Ám in panel j is valid for all photographs.