Figure 2: Akt levels are reduced in the nucleus upon activation of LXR. MCF7 cells were treated with vehicle or 5 μM GW3965 for 16 h. Cells were activated with IGF-1 (50ng/ml) for 20 min, cytoplasmic or nuclear cell extract isolated and Western immunoblotting performed with various antibodies as described in the figure. 14-3-3ζ and histone 1 antibodies were used as control for fractionation of cytoplasm and nuclease, respectively.