Figure 1: PR localization in U373 human astrocytoma cells. PR was stained by indirect immunofluorescence using FITC (green) labeled secondary antibody. Cells were treated with vehicle (V); 48 h with estradiol (E) (10 nM); E followed by progesterone (P) (10 nM) for 24 h (E+P); E followed by RU486 (PR antagonist, 1 ┬ÁM) for 24 h (E+RU); E followed by P+RU486 for 24 h (E+P+RU). Nuclei were counterstained with HOECHST. A representative assay of five independent experiments is shown.