Figure 1: A-E showed the photomigrographs of the liver of rats in the control and the experimental groups (Hand E) x 400. Normal liver hepatocyte (N) was observed in the control rat (Figure 1A) whereas hepatocyte necrosis (H) was observed in rat treated with 20 mg/kg of methotrexate (Figure 1B). Hepatocyte necrosis (H) was observed in rat treated with 10 mg/kg of melatoin + 20 mg/kg of methotrexate (Figure 1C), Also, hepatocyte necrosis (H) was observed in rat treated with 10 mg/kg of alpha lipoic acid + 20 mg/kg of methotrexate (Figure 1D) whereas normal hepatocyte (N) was observed in rat treated with melatonin + alpha lipoic acid + methotrexate (Figure 1E).