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Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 90-96
doi:10.32527/2017/101429

The Possible Modulatory Effect of Curcumin on a Rat Model of Crohn’s Disease

Rasha B. Abd-Ellatief1, Esraa A. Ahmed2, and Ebtehal A Ahmed3

1MD Lecturer of Pharmacology-faculty of Medicine-Assiut University- Assiut-Egypt.

2MD. Lecturer of Pharmacology-faculty of Medicine-Assiut University- Assiut-Egypt.

3Assistant lecturer of Pathology faculty of Veterinary Medicine -Assiut University- Assiut-Egypt

Copyright © 2017 Rasha B. Abd-Ellatief et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: Crhon’s disease (CD) is a multifactorial chronic inflammatory disease that affect the gastrointestinal tract from mouth to anus. The effect of various drugs used to treat CD was investigated. Aim: The aim of the present study is to evaluate the possible therapeutic effect of curcumin on the severity of CD induced by intra-colonic instillation of NaOH in rats. Methods: Adult male Wister rats were assigned into five groups. Group I was kept as normal animals without treatment. Group II, III, IV and V were subjected to the induction of Crhon’s disease by intra-colonic injection of 2ml NaOH (6.25%). GroupII received 0.9% saline, group III received dexamethasone at dose of (1mg/kg ), group IV and V received intraperitoneal curcumin (40,100mg/kg) once daily starting 2 days before NaOH infusion until the end of the experiment at day 15 post induction. Assessment of the inflammatory response was done by histology and measurement of interleukin-1β (IL1), tumor necrosis factor (TNF-α), myeloperoxidase activity (MPO) and reduced glutathione (GSH) levels in colon mucosa. Results: High dose of curcumin significantly decreased colonic IL-1β, TNF-α, and MPO activity and increased GSH concentration. Moreover, curcumin attenuated the macroscopic and histopathological changes induced by NaOH. Conclusion: These results suggest that curcumin may be effective in the treatment of CD through its scavenging properties on the oxygen-derived free radicals.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 90-96
doi:10.32527/2017/101429

The Possible Modulatory Effect of Curcumin on a Rat Model of Crohn’s Disease

Rasha B. Abd-Ellatief1, Esraa A. Ahmed2, and Ebtehal A Ahmed3

1MD Lecturer of Pharmacology-faculty of Medicine-Assiut University- Assiut-Egypt.

2MD. Lecturer of Pharmacology-faculty of Medicine-Assiut University- Assiut-Egypt.

3Assistant lecturer of Pathology faculty of Veterinary Medicine -Assiut University- Assiut-Egypt

Copyright © 2017 Rasha B. Abd-Ellatief et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background: Crhon’s disease (CD) is a multifactorial chronic inflammatory disease that affect the gastrointestinal tract from mouth to anus. The effect of various drugs used to treat CD was investigated. Aim: The aim of the present study is to evaluate the possible therapeutic effect of curcumin on the severity of CD induced by intra-colonic instillation of NaOH in rats. Methods: Adult male Wister rats were assigned into five groups. Group I was kept as normal animals without treatment. Group II, III, IV and V were subjected to the induction of Crhon’s disease by intra-colonic injection of 2ml NaOH (6.25%). GroupII received 0.9% saline, group III received dexamethasone at dose of (1mg/kg ), group IV and V received intraperitoneal curcumin (40,100mg/kg) once daily starting 2 days before NaOH infusion until the end of the experiment at day 15 post induction. Assessment of the inflammatory response was done by histology and measurement of interleukin-1β (IL1), tumor necrosis factor (TNF-α), myeloperoxidase activity (MPO) and reduced glutathione (GSH) levels in colon mucosa. Results: High dose of curcumin significantly decreased colonic IL-1β, TNF-α, and MPO activity and increased GSH concentration. Moreover, curcumin attenuated the macroscopic and histopathological changes induced by NaOH. Conclusion: These results suggest that curcumin may be effective in the treatment of CD through its scavenging properties on the oxygen-derived free radicals.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 7 (2017), Issue 2, pp. 90-96
doi:10.32527/2017/101429

The Possible Modulatory Effect of Curcumin on a Rat Model of Crohn’s Disease

Rasha B. Abd-Ellatief1, Esraa A. Ahmed2, and Ebtehal A Ahmed3

1MD Lecturer of Pharmacology-faculty of Medicine-Assiut University- Assiut-Egypt.

2MD. Lecturer of Pharmacology-faculty of Medicine-Assiut University- Assiut-Egypt.

3Assistant lecturer of Pathology faculty of Veterinary Medicine -Assiut University- Assiut-Egypt

Copyright © 2017 Rasha B. Abd-Ellatief et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite this Article

Rasha B. Abd-Ellatief, Esraa A. Ahmed, and Ebtehal A Ahmed, “The Possible Modulatory Effect of Curcumin on a Rat Model of Crohn’s Disease,” Egyptian Journal of Basic and Clinical Pharmacology, Vol. 7, Issue 2, pp. 90-96, 2017. doi:10.32527/2017/101429.