|Figure 2: The response of I281T mutant to CAR ligands. Activation of GAL4-driven reporter gene
by the wild-type CAR (black columns) and I281T mutant (grey columns) LBD constructs
after a 24-hour exposure to either DMSO vehicle, CAR agonists CITCO (1 μM), FL81 (10
μM), clotrimazole (CLOTR, 4 μM) or the inverse agonist S07662 (10 μM). The results
were normalized to the activity of wild-type CAR in the presence of DMSO vehicle (set
at 100). The data shown are means ± SEM of nine replicates except for FL81 (N = 6).
The symbols * and # indicate a statistically significant difference (p < 0.01) to
wild-type CAR or to the relevant DMSO control, respectively.