Table 1: Comparison of Adipocyte GR Knock Out Mouse Studies.


Mouse Design Shen et al [ 45 ] Adipoq-Cre FVB/NJ background (Jackson Laboratory, 010803), GR floxed exon 3 [ 89 ] (Jackson Laboratory, B6.Cg- Nr3c1 𝑡𝑚1.1 𝐽𝑑𝑎 /J, 021021)
Bose et al [ 46 ] Adipoq-Cre FVB/NJ background (Jackson Laboratory, 010803), GR floxed exon3 [ 89 ]
Desarzens et al [ 78 ] Adipoq-Cre FVB/NJ background (Jackson Laboratory, 010803), GR floxed exon 3 [ 88 ]
Mueller et al [ 91 ] Adipoq-Cre FVB/NJ background (Jackson Laboratory, 010803), GR floxed exon 3 [ 88 ]
de Kloet et al [ 86 ] Adipoq-Cre C57BL/6 × 129 background. GR floxed exon 2 [ 90 ]
Fasting Shen et al [ 45 ] No change in fasting plasma insulin levels in both Chow and HFD. 4 hr fast showed no difference in plasma NEFA and glycerol but reduced lipolysis under isoproterenol stimulation
Bose et al [ 46 ]
Desarzens et al [ 78 ]
Mueller et al [ 91 ] AGRKO mice after 48hr fast. 2x higher body fat mass than controls. Increased fasting blood glucose. Impaired upregulation of fasting induced gene expression. AGRKO mice eWAT had decreased phosphorylation of HSL and perilipin. AGRKO had reduced cAMP generation, NEFA release.
de Kloet et al [ 86 ]
Chow Shen et al [ 45 ] No difference in body weight or composition. No difference in glucose tolerance or insulin sensitivity
Bose et al [ 46 ] No difference in bodyweight and fat mas
Desarzens et al [ 78 ] No difference in bodyweight, fat mass, or adipocyte size. No difference in plasma free fatty acids, and cholesterol. Slight decrease in TG levels in AGRKO mice. No difference in glucose and insulin levels
Mueller et al [ 91 ] 4hr and 16 hr fasting blood glucose and plasma insulin levels showed no difference. No difference in GTT, but ITT showed delayed posthypoglycemic recovery likely due to decreased hepatic glucose production. Increased insulin induced AKT phosphorylation. Lower liver TG levels. Reduced NEFA release from 4hr and 16hr fasted AGRKO eWAT. Lower blood glucose levels during PTT in AGRKO mice
de Kloet et al [ 86 ] No difference in body weight, body mass, adiposity, and adipocyte morphology. NO difference in basal glucose levels. Unchanged adiponectin, insulin, and leptin in AGRKO mice
HFD Shen et al [ 45 ] 14 week 58% Fat diet*: AGRKO trending to gain more weight. KO has decreased plasma TG and NEFA. Small increase in Gpat4 in eWAT. In liver, trending to decreased lipid metabolism and gluconeogenic genes with Mttp being significant. GTT and ITT no difference
Bose et al [ 46 ] 16 weeks 42% Fat diet*: Similar body composition, BMD, BMC. No difference in glucose levels, GTT, nor ITT No difference in liver TG levels.
Desarzens et al [ 78 ] 15 weeks 21.2% fat, 34.5% sucrose diet*: No difference in bodyweight, fat mass, or adipocyte size. No difference in plasma free fatty acids, triglycerides, and cholesterol. No difference in glucose and insulin levels. AGRKO mice on HFSD had increased area under the curve for GTT compared to fl/fl, which remained the same. Histology of adipose showed more crown-like structures in AGRKO mice, increased lipolcalin-2 and increased activation of JNK without effects on ERK1/2
Mueller et al [ 91 ] 20 weeks 34.6% Fat diet* Reduced HFD induced obesity, decreased weight gain, reduced hepatic steatosis, improved glucose tolerance. Decreased FA and NEFA. Decreased expression of FA storage genes (Acaca, Gpat2, Dgat2, Pck1, Slc27a1 in eWAT. AGRKO mice had lower WAT weights than controls, No difference in plasma corticosterone levels, AGRKO mice had improved glucose tolerance and increased insulin sensitivity. AGRKO had decreased hepatic steatosis and decreased expression of Fasn, Gpat1, Pparq, Cd36, and Slc27a4 in liver.
de Kloet et al [ 86 ] 40% fat diet*: AGRKO mice gain significantly less weight. Decreased adipose mass, decreased energy consumption.
Exogenous GC Shen et al [ 45 ] 3mg/kg** Dexamethasone IP every other day for 2 months. No difference in body weight. Trend towards increased eWAT and iWAT. AGRKO mice more glucose and insulin tolerant than Flox mice. Improved insulin sensitivity in muscle. Decreased ability of Dex to induce whitening of BAT and Stimulated lipolysis.
Bose et al [ 46 ] 10mg/kg** Dexamethasone for 2 weeks. No difference in blood glucose levels at baseline, GTT, or ITT. No difference in body composition, but AGRKO mice had higher fat mass than WT 10mg/kg Corticosterone 4 weeks: No difference in baseline blood glucose nor GTT and ITT. No difference in bodyweight, fat mass, BMD, nor liver TG levels
Desarzens et al [ 78 ]
Mueller et al [ 91 ]
de Kloet et al [ 86 ] 0.1mg/kg** Dexamethasone injected AGRKO mice showed under stress an impaired negative feedback of HPA
BAT Shen et al [ 45 ] Cold exposure showed lower glycerol and NEFA levels in AGRKO mice.
Bose et al [ 46 ] 10mg/kg acute Dexamethasone for 6 hrs resulted in upgregulation of some, but not all metabolism genes in AGRKO mice BAT.
Desarzens et al [ 78 ]
Mueller et al [ 91 ] 4hr fast followed by 4h 4 C. Ability to maintain body temperature at 4 C was reduced. Lipid droplet decreased more in AGRKO BAT. Decreased cold induced thermogenesis due to impaired lipolysis and NEFA flux.
de Kloet et al [ 86 ]
HPA Axis Shen et al [ 45 ]
Bose et al [ 46 ] Dexamethasone suppression test showed decrease in AGRKO mice after Dexamethasone injection showing HPA axis activation was not altered.
Desarzens et al [ 78 ]
Mueller et al [ 91 ]
de Kloet et al [ 86 ] Similar baseline corticosterone levels. Under stress, AGRKO mice had increased corticosterone and hyperglycemia

Note: *High fat diet use in the papers ranged from 21%-58% fat. **Dexamethasone doses ranged from 0.1mg/kg to 3mg/kg and varied in duration from acute to chronic exposure. These high fat diet percentages as well as doses of dexamethasone could be responsible for the differences observed by the papers.