Table 4: Secondary structures of hLXRα and hPPARα proteins in the absence of ligands a .


Proteins α-helix regular H(r)% α-helix distort H(d)% β-sheet regular S(r)% β-sheet distort S(d)% Turns T% Unordered U%

LXRα 29.7 ± 1.0 23.3 ± 0.8 10.0 ± 1.0 11.0 ± 1.0 14.3 ± 2.0 11.0 ± 2.0
PPARα 28.0 ± 0.0 19.0 ± 1.0 9.3 ± 1.0 8.0 ± 1.0 14.3 ± 0.6 20.3 ± 2.0
PPARα/LXRα (Obs) 32.3 ± 1.2 b 25.7 ± 0.6 b 8.3 ± 0.6 8.0 ± 1.0 12.3 ± 0.3 13.0 ± 2.0
PPARα/LXRα (Calc) 27.0 ± 0 22.0 ± 1.0 8.0 ± 0.0 10.0 ± 0.0 15.0 ± 1.0 17.5 ± 1.5
PPARα/LXRα H383E (Obs) 24.0 ± 2.0 16.0 ± 1.0 10.5 ± 0.5 b 8.0 ± 0.0 17.5 ± 1.5 24.5 ± 0.5 b
PPARα/LXRα H383E (Calc) 21.5 ± 1.5 17.5 ± 1.5 14.0 ± 0.5 10.5 ± 3.5 17.0 ± 2.0 19.5 ± 0.6
PPARα/LXRα E387Q (Obs) 28.0 ± 0.0 b 22.3 ± 0.6 8.6 ± 0.3 8.0 ± 1.0 b 13.7 ± 0.3 19.9 ± 1.5 b
PPARα/LXRα E387Q (Calc) 23.5 ± 0.5 23.5 ± 0.5 9.5 ± 1.5 14.0 ± 0.0 15.0 ± 1.0 13.5 ± 0.5
PPARα/LXRα H390E (Obs) 31.5 ± 0.5 b 17.0 ± 1.0 10.0 ± 0.0 9.0 ± 1.0 12.0 ± 2.0 20.5 ± 0.5 b
PPARα/LXRα H390E (Calc) 25.5 ± 0.9 15.8 ± 0.8 9.5 ± 1.1 8.7 ± 0.7 14.5 ± 0.6 25.2 ± 0.5
PPARα/LXRα L414R (Obs) 26.7 ± 0.8 19.3 ± 0.8 7.6 ± 0.3 b 6.6 ± 0.3 16.0 ± 0.5 23.3 ± 0.8 b
PPARα/LXRα L414R (Calc) 27.5 ± 0.5 18.0 ± 0.0 5.5 ± 0.5 6.0 ± 0.0 16.5 ± 0.5 26.0 ± 0.0
PPARα/LXRα R415A (Obs) 29.2 ± 0.8 23.4 ± 0.8 10.8 ± 0.5 b 8.0 ± 0.6 12.8 ± 0.9 16.4 ± 1.3
PPARα/LXRα R415A(Calc.) 30.5 ± 0.5 23.5 ± 1.5 7.0 ± 1.0 8.5 ± 3.5 13.0 ± 2.0 16.5 ± 4.5

a Definitions: Obs, obtained experimentally; calc, calculated average. Significant difference between observed and calc for each protein mixture ( n = 4-6). b p < 0.05.