Retinoic Acid-Related Orphan Receptors (RORs): Regulatory Functions in Immunity, Development, Circadian Rhythm, and Metabolism

In this overview, we provide an update on recent progress made in understanding the mechanisms of action, physiological functions, and roles in disease of retinoic acid related orphan receptors (RORs). We are particularly focusing on their roles in the regulation of adaptive and innate immunity, brain function, retinal development, cancer, glucose and lipid metabolism, circadian rhythm, metabolic and inflammatory diseases and neuropsychiatric disorders. We also summarize the current status of ROR agonists and inverse agonists, including their regulation of ROR activity and their therapeutic potential for management of various diseases in which RORs have been implicated.


RORs in Adaptive Immunity
The innate and adaptive immune systems are highly integrated and serve to protect the host from being overwhelmed by pathogen invasion. Innate immune responses are immediate and utilize germline-encoded receptors to recognize and respond to pathogens, whereas adaptive immunity is a delayed response that requires expansion of a small number of cells bearing antigen-specific receptors on the surface of lymphocytes. Genetically modified mice lacking RORα or RORγt have revealed that each receptor plays a key role in the development of several immune cells and are critical for some immune responses (Figure 1).
Lymphoid progenitor cells undergo several stages of differentiation in the thymus prior to becoming mature T cells. These stages can be identified in part by display of CD4 and CD8 on the cell surface. RORγt is selectively expressed in T cells that display both CD4 and CD8, typically called double positive (DP) cells. RORγt is required in these cells for expression of the anti-apoptotic gene Bcl-X L [36][37][38][39][40]. Bcl-X L expression is repressed in DP thymocytes of RORγ null mice, resulting in accelerated apoptosis in vivo and in vitro. Consequently, thymi of RORγ null mice have reduced numbers of DP cells and their descendants, including single positive (SP) mature CD4 + T helper cells (Th) and CD8 + cytotoxic cells.
cell signaling axis has also been linked to the control of Th17 differentiation by RORγt [56]. Activation of PI3K-Akt-mTORC1 induces ribosomal protein S6 kinase β2 (RPS6KB2) expression that subsequently promotes the nuclear localization of RORγt and RORγtmediated Th17 differentiation.
The differentiation of Th0 cells into Treg and Th17 cells is dependent on the balance between the level of expression of Foxp3 and RORγt, respectively. Through its interaction with RORγt, Foxp3 inhibits RORγt function and promotes Treg differentiation [1,57,58]. This balance is controlled by the concentration of specific cytokines in the environmental milieu. Foxp3 expression and consequent Treg development is favored in cultures containing high levels of TGF-β, IL-2 and retinoic acid, whereas Th17 development is promoted by low amounts of TGF-β in combination with the proinflammatory cytokines, IL-6 and IL-1 [58][59][60]. IL-1 can repress the suppressor of cytokine signaling 3 (SOCS3), an inhibitor of STAT3 phosphorylation [61], thereby increasing Rorc expression. Th17 cells share an overlapping developmental program with that of inducible regulatory T cells (iTregs) [62]. In the small intestine, a number of RORγt + Foxp3 + T cells do not produce IL-17, but instead express IL-10. These RORγt + Tregs develop outside the thymus and require gut microbiota for their development. In addition, dietary vitamin A favors the generation of these RORγt + Tregs over that of Th17 cells [62]. RORγt + Treg cells regulate Th2 cells -but not Th1 or Th17 cells -through a CTLA4-dependent regulation of CD80 and CD86 on dendritic cells.
Th17 and IL17 have been implicated in several inflammatory and autoimmune diseases. Mice lacking RORγ are partially protected against the development of diseases, including autoimmune diseases such as experimental autoimmune encephalomyelitis (EAE) and type II collagen-induced arthritis, as well as allergen-induced lung inflammation [12,44,58,63]. Mice lacking both RORα and RORγ are greatly protected from EAE [44]. Although IL-17A, IL-17F and IL-22 are the signature cytokines of Th17 cells, they appear not to be sufficient for pathogenicity in EAE [64,65]. In this model, RORγt-dependent production of granulocyte macrophage colony stimulating factor (GM-CSF) is reported to drive the effector phase of neuroinflammation [66,67]. However, the molecular requirements of pathogenicity might depend on the disease model because either IL-17A or IL-17F is required for Th17 cell-mediated intestinal inflammation [68]. Together, these studies raise the possibility that RORγ antagonists might be useful in the management of autoimmune disease.

RORs in Innate Immunity
Like conventional αβ T cell receptor (TCR) + cells, T cells expressing the γ and δ TCR chains (γδ T cells) develop in the thymus, but they have a more limited repertoire than αβ TCR + cells and lack major histocompatibility complex (MHC) restriction [69]. Many γδ T cells express IL-17 and are thus termed γδ-17 T cells [70,71]. Unlike αβ TCR Th17 cells, which acquire effector functions only after encountering their cognate antigens in peripheral tissue, many γδ-17 cells express IL-17 very early during their development in the thymus, even prior to TCR rearrangement [70,71]. Thus, γδ-17 cells have the potential to be a major of antigen-specific Th17 cells [14,72]. There are some commonalities, but also differences in the molecular pathways leading to γδ-17 and Th17 cells. For both cell types, RORγt is critically important. Thus, mice deficient for RORγt lack γδ-17 cells in peripheral organs and lymphoid tissues [12]. However, the induction of RORγt in Th17 cells requires the canonical c-Rel-dependent NF-kB pathway, whereas γδ-17 cells require RelB and the noncanonical NF-kB pathway [73]. In addition, IRF4 is required for the induction of Th17 cells, but this transcription factor is dispensable for the development of γδ-17 T cells.
RORα and RORγ also play a critical role in the generation of innate lymphoid cells (ILCs). ILCs are a heterogeneous population of cells that possess the typical lymphoid cell morphology, but lack some cell surface molecules typically seen on lymphocytes [74]. In particular, ILCs lack TCRs and the associated CD3 complex found on conventional T cells. Consequently, ILCs cannot recognize specific antigens, and instead respond to cytokines produced during innate immune responses. ILCs have been classified into three groups, based on their cytokine production profiles and the transcription factors that regulate their development [75]. The cytokines produced by each of these groups mirrors those produced by specific T helper (Th) cell types: Group 1 ILCs and Th1 cells produce IFN-γ, Group 2 ILCs and Th2 cells produce IL-5 and IL-13, and Group 3 ILCs and Th17 cells produce IL-17A, IL17F and IL-22.
RORγt is required for the development of all ILC3s, a heterogeneous population of cells that also depends on IL-7 for their development. The first discovered member of the Group 3 ILCs is the lymphoid tissue inducer (LTi) cell, a type of CD4 + CD3 − cell that displays transmembrane lymphotoxin α1β2 [76,77]. These cells are required for the development of secondary lymphoid organs, Peyer's patches, and intestinal lymphoid follicles [78][79][80][81]. RORγt-deficient mice lack these cells and therefore do not develop secondary lymphoid organs [1,36,37,82]. Recently, retinoic acid (RA) was found to control LTi cell maturation upstream of RORγt by positively regulating RORγt expression directly through the recruitment of RA receptors (RARs) to the promoter region of RORγt [83]. Impairment of LTi maturation in cells defective in RA signaling can be rescued by the exogenous expression of RORγt. More recently, other ILC3 subpopulations have been identified that, like Th17 cells, are abundant in the gut and can produce IL-17A, IL-17F, IL-22, GM-CSF, and TNF, suggesting their importance for clearing extra-cellular pathogens. Gata-3 is critical for the development of gut RORγt + ILC3s subsets [84]. At least some ILC3s can transition to ILC1 cells [85], reminiscent of Th17 cell conversion to a Th1-like phenotype. This transition, which is accompanied by elevated levels of TBX21, is driven by cytokine signals in the cellular milieu.
Group 2 ILCs are the most homogeneous group of the ILCs and are dependent on RORα, but not RORγt, for their development [86,87]. They display the cell surface markers IL-7Ra (CD127), IL-2RA (CD25), Sca-1, KLRG1 and the IL-33 receptor, ST2. ILC2s are defined based on their ability to produce an array of type 2 cytokines, including IL-4, IL-5, IL-9, and IL-13, as well the cytokine, amphiregulin, and are implicated in helminth clearance and allergic inflammation [88]. Several single nucleotide polymorphisms (SNPs) within the RORA gene are associated with increased susceptibility to asthma [89][90][91], and RORα null mice and ILC2-deficient mice generated by RORα-deficient bone marrow transplants have reduced type 2 cytokine production and partial protection from airway hyper-reactivity [87,92]. RORα expression was significantly upregulated in patients with therapy-resistant asthma [93]. RORα expression was also found to be significantly elevated in skin from patients with atopic dermatitis (AD), while in an experimental model of AD-like inflammation RORα-deficient mice exhibit a profound deficit in ILC2 cells and significantly reduced allergic skin inflammation [88]. Together, these observations indicate that RORα plays a critical role in ILC2 cell lineage determination and control of allergy-induced inflammation in multiple tissues.
RORα also contributes to immune function in the intestinal epithelium by controlling the diurnal regulation of several pathogen recognition receptors, including Nod2 and various Toll-like receptors [94]. The expression of these genes in the intestine is reduced in RORαdeficient mice, particularly when RORα expression is at its highest and bound to promoter regions of these genes. Other genes whose diurnal expression is directly controlled by RORα include interleukin-1 receptor-associated kinase 1 (IRAK1), Toll-interleukin 1 receptor domain containing adaptor protein (TIRAP), and the clock genes, Bmal1 and nuclear factor, interleukin 3 regulated (NFIL3 or E4BP4). The decreased expression of RORα in the intestine of microbiota-depleted mice further provides additional evidence of a relationship between RORα, commensal bacteria and diurnal regulation of immune-related genes in the gut [94].

ROR Functions in Brain and Retina
RORα is highly expressed and developmentally regulated in several regions of the brain, including cerebellar Purkinje cells and the thalamus [1,[95][96][97]. Genetically modified mice, in which RORα is disrupted, and RORα-deficient staggerer (RORα sg/sg ) mice display severe cerebellar ataxia due to cerebellar neurodegeneration [98][99][100]. Further characterization of these mice revealed that RORα plays a critical role in the regulation of the survival and differentiation of Purkinje cells from embryonic development throughout adulthood [98,[100][101][102][103]. Deletion of RORα in Purkinje cells between postnatal days 10-21 revealed that continued expression of RORα is necessary after neuronal maturation to maintain mature morphological and innervation characteristics in the adult Purkinje cells [103]. Loss of Purkinje cells was also observed in >6 months-old male RORα +/sg mice and to a much smaller degree in RORα +/sg females. The marked, age-related Purkinje cell death in RORα +/sg male mice has been linked to a premature decrease in circulating sex steroids, which have been shown to be neuroprotective, and appeared not to be due to changes in cerebellar neurosteroids [104]. Genome-wide gene expression studies showed that RORα regulates the expression of a number of genes linked to Purkinje cell maturation, particularly dendritic differentiation, and the glutamatergic pathway [98]. ChIP analysis demonstrated that RORα directly controls the transcription of several of these genes, including the glutamate transporter Slc1a6, the calmodulin inhibitor Pcp4, and the IP3 receptor (Itpr1). Several of the RORα target genes were found to be also down-regulated in Ski family transcriptional corepressor 2 (Skor2)-deficient mice; however, RORα expression was not altered in these mice [105]. It was proposed that RORα and Skor2 cooperate in regulating Purkinje cell differentiation and gene expression. In Purkinje cells, RORα also directly regulates the expression of sonic hedgehog (Shh) [98], which is required for the proliferation and survival of cerebellar granule precursor cells through its activation of Gli transcription factors. The degeneration of cerebellar granule cells observed in RORα-deficient mice can at least in part be attributed to this loss in Shh production.
Accumulating evidence indicates a role for RORα in several neuropsychiatric disorders, including autism spectrum and bipolar disorder (ASD), schizophrenia, depression, and posttraumatic stress syndrome [106][107][108][109][110][111][112][113][114][115][116][117]. Studies demonstrating that the expression of RORA was reduced in sections of cerebellum and cortex of autistic subjects and observations showing differential methylation of the RORA gene in lymphoblastoid cells from autistic and nonautistic siblings supported a role of RORα in the development of ASD [107]. This, together with reports showing reduced number and size of Purkinje cells (PC) in the majority of cerebellar specimens from ASD patients, as was observed in RORα-deficient mice (Chugani 2014), is consistent with a link between RORα, its regulation of Purkinje cell survival and differentiation, and ASD. A connection between RORα and ASD is further supported by genome-wide ChIP-Seq analysis showing that in human neuronal cells, RORα was associated with the promoter region of a number of ASD-associated genes, including ataxin binding protein (A2BP1), neuroligin 1 (NLGN1), and aromatase (CYP19A1) [118,119]. Several of the genes down regulated in RORα-deficient neuronal cells, including aromatase, were also found to be repressed in the frontal brain of individuals with ASD. The positive regulation by RORα of aromatase, which converts testosterone to estrogen, is intriguing because estrogen has been reported to enhance RORα expression and to exhibit a neuroprotective effect, as reported for RORα. Thus, RORα, aromatase, and estrogen might be part of a positive regulatory pathway. Therefore, reduced expression of aromatase and estrogen production in RORα-deficiency might excerbate Purkinje cell death and enhance the risk for ASD. Loss of Purkinje cells was also observed in >6 months-old male RORα +/sg mice and to a much lesser degree in RORα +/sg females. The marked, age-related Purkinje cell death in RORα +/sg male mice was linked to a premature decrease in circulating sex steroids, which have been shown to be neuroprotective, and not due to changes in cerebellar neurosteroids [104]. Since both RORα and estrogen exhibit neuroprotective effects, this pathway might help to explain why RORα +/sg female mice are less susceptible to Purkinje cell loss during aging. Further indications for a link between RORα and ASD came from a recent study demonstrating that the microRNA MIR137, which has been implicated in ASD and schizophrenia, targets the 5'-UTR of RORα [108]. RORα has also been reported to have neuroprotective functions in neurons and astrocytes during hypoxia [120,121]. Protecting brain cells from the damaging effects of injury and stress might be an important function of RORα and be relevant to several brain disorders.
RORβ is highly expressed in the suprachiasmatic nucleus, the retina and pineal gland, and has been implicated in the regulation of circadian, motor, and visual functions [3, 122,123]. RORβ deficient mice displayed motor ("duck gait," hind paw clasping reflex), olfactory deficits, reduced anxiety and learned helplessness-related behaviors, and alterations in circadian behavior [124]. Examination of RORβ expression during embryonic and postnatal development of the mouse neocortex showed that after E16.5 RORβ transcripts increasingly localized to the primary sensory areas and reached peak expression at P10 with strongest expression in the primary somatosensory, auditory, and visual areas [125,126]. This developmental pattern of expression was similar to that reported for rat neocortex [127]. A possible connection was found between RORβ expression levels and the control of cytoarchitectural patterning of neocortical neurons during mouse development [128]. GWAS studies revealed an association between a series of RORB genetic variants with schizophrenia, and bipolar I disorder [113,116,129,130] and between RORβ expression in the temporal cortex and verbal intelligence [131]. Similarly, a syndrome characterized by moderate facial dysmorphy, mental retardation, epilepsy, speech delay, and autistic behaviour in patients with a 9q21 deletion at the RORB locus identified RORβ as a strong candidate for this neurological disorder [132][133][134]. Prenatal ethanol exposure has been reported to lead to neurobiological damage in early development. Newborns prenatally exposed to alcohol show neuroanatomical defects in the neocortex and an abnormal neocortical expression pattern of RORβ that is associated with mental and intellectual dysfunction, and behavioral and motor deficits [135].
Recent studies showed that the RORβ1 and RORβ2 isoforms exhibit distinct patterns of expression during retinal development [136,137]. RORβ was shown to play a critical role in regulating retinal progenitor proliferation and differentiation [123,[137][138][139]. RORβ1deficient mice lack amacrine and horizontal interneurons, cells important for the integration of visual information, suggesting that RORβ1 is critical for the differentiation of retinal progenitors into these interneurons [136]. This involves direct transcriptional regulation of Ptf1a, a key factor required for the generation of amacrine and horizontal cells, by RORβ1. Re-expression of RORβ1 was able to rescue amacrine differentiation in RORβ null mice. Retinal progenitors can also differentiate into two morphologically, developmentally, and functionally distinct photoreceptors, rods and cones. Rods function in dim light, while cones mediate daylight, and in most mammals, color vision. Mice lacking RORβ lose rods, but overproduce primitive S cones that lack outer segments [139]. RORβ1 and RORβ2 control rod cell differentiation through its transcriptional regulation of neural retina leucine zipper factor (NRL), a transcription factor that promotes the differentiation of retinal progenitors into the rod cell lineage, while suppressing the cone cell lineage [137,139,140]. The lack of rod photoreceptors in RORβ null mice is in part due to the loss of NRL expression. This can be reversed by re-expression of NRL in these mice [139]. RORβ2 is expressed in rod photoreceptors and its transcription was shown to be directly regulated by NRL. Thus, RORβ2 and NRL form two positive feedback loops that synergistically promote the commitment to a rod cell lineage [137]. In addition to these roles, RORβ-deficient mice fail to induce S opsin appropriately during postnatal cone development, suggesting a function for RORβ in morphological maturation of cone photoreceptors [123]. RORβ was found to activate the S opsin gene (Opn1sw) expression through binding sites in the upstream promoter region.

Role of RORs in Cancer
Very little is known about the role of RORβ and RORγ in cancer. Initial analysis of RORγ knockout mice revealed that these mice develop T-cell lymphomas within the first months after birth that rapidly metastasize to liver and spleen [141]. The mechanism underlying the development of thymic lymphomas in RORγ null mice has yet to be elucidated. A recent study reported that low levels of RORγ mRNA expression in somatotroph adenomas was associated with reduced E-cadherin expression and increased epithelial mesenchymal transition (EMT), and increased tumor size and invasiveness [142]. Similarly, higher expression of RORγ correlated with longer metastasis-free survival in breast cancer [143]. With respect to RORβ and cancer, a recent study reported that RORB is overexpressed in primary leiomyosarcomas, the most common type of uterine sarcoma [144]. In contrast, RORB expression was found to be highly down-regulated in both serous and endometrioid types of endometrial cancer [145].
A number of studies reported that RORα expression is significantly down-regulated during tumor development and progression, while expression of exogenous RORα inhibited cell proliferation and tumor growth [146][147][148][149][150][151]. Reduced RORα expression has been observed in colorectal and mammary carcinomas and found to be associated with poorer prognosis in hepatocellular and breast carcinoma patients [146,147,150,152,153]. In addition, silencing RORα in mammary epithelial cells significantly enhanced cell proliferation in ductal epithelial cells and promoted side branching of mammary ducts, suggesting that RORα has an important role in mammary gland branching morphogenesis [150]. Conversely, restoring RORα expression in cultured breast cancer cells was shown to inhibit cell migration and suppress tumor growth and metastasis in nude mice. This was accompanied by enhanced expression of semaphorin 3F (SEMA3F), a tumor suppressor that inhibits tumor growth and invasiveness. RORα was shown to regulate SEMA3F transcription directly through ROREs in its promoter region [150]. A different study indicated a role for SPARC, which is critical in the regulation of cell growth and adhesion, in the anti-tumor and anti-proliferation effects of RORα in human hepatoma cells [154]. SPARC was found to be a direct target gene of RORα. Treatment of colon carcinoma HCT116 cells with DNA-damage agents led to a p53dependent increase in RORα expression that is directly mediated through functional p53 response elements in the RORa promoter [155]. RORα itself stabilized p53 by inhibiting its ubiquitination and enhanced p53 transcription in a HAUSP/Usp7-dependent manner leading to increased apoptosis. The connection between RORα and p53 was supported by a report demonstrating that treatment of hepatocellular carcinoma cells with an RORα agonist enhanced p53 stability [156]. This increase was shown to involve elevated SOX4 transcription, a gene critically involved in MDM2-dependent regulation of p53 stability. Another role for RORα in cancer was revealed by a recent study demonstrating that RORα expression was decreased in tumor tissues compared to adjacent normal tissues in human hepatocellular carcinoma patients and that this was associated with a change in glucose metabolism [157]. RORα was shown to inhibit pyruvate dehydrogenase kinase 2 (PDK2) expression and phosphorylation, thereby promoting aerobic glycolysis rather than oxidative phosphorylation, whereas reduced RORα expression in tumor cells promotes oxidative phosphorylation and tumor cell growth.
Several studies revealed a role for a noncanonical RORα pathway in cancer that does not involve RORE binding, but in which RORα functions as a transcriptional cofactor. In colon carcinoma cells, RORα was shown to bind β-catenin directly and inhibit β-catenin-mediated transcriptional activation of the target genes, cyclin D1 (CCND1) and c-myc (MYC), resulting in repression of cell proliferation and migration [158]. RORα was found to be recruited to the lymphoid enhancer-binding factor 1 (LEF1)-binding sites of the LEF1-target gene, CCND1, together with LEF1 and β-catenin. RORα interacted with residues within the armadillo repeat domains of β-catenin, which function as binding sites for a subset of coactivators. The interaction of RORα with β-catenin required the N-terminus of RORα and was dependent on the phosphorylation of Ser35 by protein kinase Cα (PKCα) activated by the noncanonical Wnt pathway. Interestingly, in many colorectal carcinomas phosphorylation of RORα was reduced. In another noncanonical pathway, RORα interacts with the heptad repeat and marked box region of the transcription factor E2F1 and suppressed E2F1-regulated transcription and cell cycle progression in epithelial cells [159]. In mammary ducts, RORα levels inversely correlated with the expression of E2F1 target genes and cell proliferation. Binding of RORα was shown to inhibit E2F1 acetylation and its DNA-binding activity by enhancing its interaction with histone deacetylase 1 (HDAC1). Knockdown of HDAC1 or inhibition of HDAC activity partially reversed the repression of E2F1 activity by RORα. In contrast to its growth inhibitory effects, RORα was shown to enhance the proliferation in mammary carcinoma MCF7 cells and significantly induced the expression of aromatase mRNA by binding an RORE in the aromatase promoter region [160]. It was proposed that the increase in aromatase expression by RORα accelerates the local production of estrogen, which then enhances the proliferation of breast cancer cells.

RORs in the Regulation of Metabolism
Both RORα and RORγ have been implicated in the control of energy homeostasis and the regulation of several lipid and glucose metabolic genes [3, 22, 24, [161][162][163][164][165][166][167][168][169]. Regulation of energy homeostasis is a complex process that involves multiple interrelated glucose and lipid metabolic pathways in many organs and is controlled by the circadian clock, gut microbiota, and by the endocrine, immune and nervous systems [170][171][172][173]. This has made it difficult to determine whether the metabolic changes observed in ROR-deficiency are cause or effect. RORα-deficient (staggerer) mice were shown to be protected against high fat diet (HFD)-induced metabolic syndrome as indicated by reduced weight gain, adiposity and hepatic steatosis, and improved insulin sensitivity [161,174,175]. Adipocytes in RORαdeficient mice fed a high fat diet accumulated considerably less lipid and the infiltration of inflammatory macrophages and expression of several inflammatory genes, including interleukin 6 (Il6), Toll-like receptor 8 (Trl8), and chemokine (C-C motif) ligand 8 (Ccl8), were greatly diminished [174]. Interleukin-1 receptor antagonist (Il1rn) was among the genes most dramatically repressed in white adipose tissue (WAT) of RORα-deficient mice. This gene has been implicated in the regulation of obesity and insulin resistance, suggesting that the reduced susceptibility to metabolic syndrome in RORα-deficient mice might at least in part be attributed to Il1rn repression [174,176]. WAT-associated inflammation plays a critical role in the development of metabolic syndrome [172,177]. The reduced inflammation observed in RORα-deficient mice might be in part responsible for the improved insulin sensitivity in these mice. A role for RORα in the regulation of insulin sensitivity is supported by a study showing an association between a single nucleotide polymorphism in RORα (rs7164773) and an increased risk for type 2 diabetes in the Mexico Mestizo population [178]. A recent study showed that the expression of several thermogenic genes, such as uncoupling protein 1 (Ucp1) and deiodinase 2 (Dio2), markers of brown adipose tissue (BAT), was enhanced in adipose tissue from RORα-deficient mice. This was associated with increased expression of the histone-lysine N-methyltransferase 1 (Ehmt1), a gene that controls BAT specification and maintenance [175,179]. The greater cold-tolerance of RORα-deficient mice appears to be related to the increased expression of these genes, leading to increased oxygen consumption and heat generation from lipid oxidation that likely contributes to the improved energy homeostasis and insulin-sensitivity observed in these mice. Both RORα and RORγ have been shown to be induced during adipocyte differentiation in 3T3-L1 cells [180]; however, exogenous expression of RORα inhibits adipocyte differentiation in 3T3-L1 cells, as indicated by the reduced induction of fatty acid binding protein 4 (Fabp4), perilipin 1 (Plin1) and fatty acid synthase (Fasn) [181].
In addition to WAT, loss of RORα induces changes in gene expression in macrophages and liver. Disruption of RORα in macrophages leads to diminished expression of cholesterol 25hydroxylase (Ch25h), which converts cholesterol to 25-hydroxycholesterol, and reduced phagocytosis [182,183]. Interestingly, addition of 25-hydroxycholesterol was able to reverse the inhibition of phagocytosis in RORα-deficient macrophages suggesting a link between oxysterol metabolism and the regulation of phagocytosis. In the liver, the expression of a large number of genes related to lipid and glucose metabolism were found to be down-regulated in RORα-deficient mice fed a HFD [174]. These included phosphoenolpyruvate carboxykinase (Pepck) and glucose-6 phosphatase (G6pc), which play a role in gluconeogenesis, fibroblast growth factor 21 (Fgf21), which is an important regulator of glucose and lipid homeostasis, and genes involved in triglyceride synthesis and storage, such as glycerol-3-phosphate acyltransferase (Gpam), perilipin 2 (Plin2), monoacylglycerol O-acyltransferase 1 (Mogat1), and cell death-inducing DFFA-like effector a (Cidea) [154,166,174,184]. In addition, the hepatic expression of several genes involved in sterol and bile acid metabolism, including cytochrome P450 8b1 (Cyp8b1), Cyp7b1, and sulfotransferase Sul1b1 were significantly diminished in RORα-deficient mice [153,167,174,185]. However, the hepatic expression of sulfotransferase Sult1e1 was found to be dramatically induced in both male and female RORα-, but not in RORγ-deficient mice, whereas Sult2a1, known to sulfonate bile acids, hydroxysteroid dehydroepiandrosterone, and related androgens, was increased in both RORα-and RORγ-deficient mice, but only in female mice [167]. In contrast, in cultured human hepatocytes and hepatoma HepG2 cells, exogenous expression of RORα induced SULT2A1, while RORα knockdown with siRNAs decreased its expression [153]. Moreover, overexpression of RORα inhibited LXR and SREBP expression as well as lipid accumulation in these cells [186]. Adenovirus-mediated overexpression of RORα in liver also reduced triglyceride levels in mice fed a high fat diet. The cause of the discrepancy between the observations in RORα-deficient mice and those in HepG2 and liver overexpressing RORα has yet to be understood. ChIP and promoter analysis indicated that many metabolic genes, including G6pc, Adfp, Cyp7b1, citrate synthase (Cs), Cyp2c8, Fgf21, secreted protein, acidic, cysteine-rich (Sparc), Sult1b1, and Sult2a1, were directly regulated by RORα in HepG2 cells [153,154,166,174,185,187,188]. RORα cistrome data [165] revealed that in liver, RORα was recruited to ROREs in several genes important in glucose homeostasis and lipid metabolism, including G6pc, Fasn, Pepck1, Apoa1, and Elovl3, indicating that RORα positively regulates the transcription of these metabolic genes by binding ROREs in their regulatory region.
RORα-deficient mice also display metabolic changes in skeletal muscle that are accompanied by alterations in the expression of several genes [169]. Glucose uptake in skeletal muscle of RORα-deficient mice was enhanced and found to be associated with increased phosphatidylinositol 3-kinase signaling and Glut4 expression [161,169]. Expression of a dominant-negative RORα in skeletal muscle C2C12 cells and in skeletal muscle in mice was reported to down-regulate the expression of carnitine palmitoyltransferase-1 (Cpt1), caveolin 3 (Cav3), and Abca1 encoding proteins involved in β-oxidation and cholesterol homeostasis, and of Srebp1c and its downstream targets, Fas and Scd1/2l, which are involved in lipogenesis [163,189]. Promoter analysis indicated that Cav3 and Cpt1 were directly regulated by RORα. Expression of a dominant-negative RORα in skeletal muscle induced mild hyperglycemia and glucose intolerance and attenuated insulin-mediated phosphorylation of Akt2. The latter contrasts with the increase in Akt2 expression and phosphorylation observed in RORα-deficient sg/sg mice.
RORγ also plays a role in the regulation of glucose metabolism and insulin sensitivity [164,165,168,190,191]. RORγ-deficient mice were significantly more insulin sensitive and glucose tolerant than WT mice. The euglycemic clamp test revealed that hepatic glucose production was considerably reduced in RORγ-deficient mice, whereas ectopic expression of RORγ in RORγ-deficient liver tissue or primary hepatocytes increased glucose production [165]. Moreover, the conversion of exogenously administered pyruvate to glucose was significantly lower in RORγ −/− . The reduced hepatic gluconeogenesis in RORγ-deficient mice may be at least partly responsible for the improved insulin sensitivity and glucose tolerance observed in these mice [165,190]. Loss of RORγ significantly decreased peak expression of several glucose (e.g., G6pase, Pklr, Glut2, PPARδ) and lipid (e.g., Insig2a, Elovl3, Cyp8b1, Sult1e1) metabolic genes [165,167,168,192]. Conversely, exogenous expression of RORγ in RORγ −/− liver tissue by adenovirus significantly increased the expression of G6pase, Pepck, Gck, Gckr, Pparδ, Pcx, and Klf15. [165]. Together, these observations indicated that RORγ is an important modulator of hepatic gluconeogenesis and glycolysis. ChIP-Seq analysis not only uncovered the consensus sequence of the in vivo RORE, but also revealed that RORγ is recruited to the regulatory region of a number of metabolic genes involved in glycolysis and gluconeogenesis, including G6pase, Pepck, Pklr, Pparδ, Gck, Gckr, Glut2, Gys2, Dlat, Pcx, and Klf15 [165]. These data indicated that RORγ positively regulates the transcription of these metabolic genes by binding ROREs in their regulatory region. Promoter analysis further supported that the expression of several of these genes was directly regulated by RORγ. The observations further suggested that the decreased expression of these genes is at least in part responsible for the reduced gluconeogenesis and lower glycogen accumulation and consequently for the improved insulin sensitivity and glucose tolerance observed in RORγ null mice. A role for RORγ in the regulation of insulin resistance is supported by studies showing that the level of RORγ expression positively correlates with adiposity and insulin resistance in human obese patients [190,191]. These observations suggest that RORγ antagonists might be beneficial in controlling glucose homeostasis and in the management of metabolic diseases.
In addition to gluconeogenesis, RORγ regulates hepatic lipid metabolism. Loss of RORγ reduced the expression of a number of lipid metabolic genes, including the insulin-induced gene 2a (Insig2a), elongation of very long chain fatty acids-like (Elovl3), Sult2a1, Cyp7b1, and Cyp8b1 [153,167,168,185]. ChIP and promoter analysis showed that several of these genes are directly regulated by RORγ. The changes in the expression of these genes were associated reduced levels of triglycerides, cholesterol, and bile acids in liver and blood in RORγ-deficient mice fed a HFD. Lipid and glucose metabolic genes are under a complex control and involve regulation by other transcription factors, including several nuclear receptors, such as Rev-Erb, PPAR, LXR, and CAR. Since some of these receptors interact with similar binding sites, the transcriptional control of several of lipid and glucose metabolic genes likely involves interplay between different nuclear receptor signaling pathways. The best known example of this is the competition of Rev-Erbs with RORs for the same binding sites. Comparison of the RORα and RORγ cistromes from liver indicated that although many genes were selectively regulated by either RORα or RORγ, several genes, including G6pc, Apoa2, Elovl5, and Cry1, were regulated by both RORα and RORγ, indicating some redundancy between the two RORs in regulating these genes [165].

RORs and Circadian Rhythm
It has been well established that the regulation of the circadian rhythm is interconnected with the diurnal control of behavior, metabolic activities, immune responses, and many other physiological functions. For example, the circadian clock has been shown to regulate the diurnal expression of many lipid and glucose metabolic genes as well as immune response genes [170,[193][194][195]. It therefore not surprising that disruption of the circadian rhythm has been linked to increased risk for metabolic diseases, including obesity, diabetes, and liver steatosis, as well as several inflammatory and neuropsychiatric disorders [170,171,[196][197][198][199][200][201]. In mammals, the suprachiasmatic nucleus (SCN) serves as the central circadian pacemaker that integrates light-dark cycle input and synchronizes the autonomous oscillators in peripheral tissues [170,171,196,197]. The molecular clock machinery consists of several transcription/translation feedback loops in which the heterodimeric complex consisting of brain and muscle ARNT-like (Bmal1) and circadian locomotor output cycles kaput (Clock) or its paralog neuronal PAS domain protein 2 (Npas2) form the positive regulatory loop of the oscillator, whereas two cryptochrome (Cry) and three period proteins (Per) are part of the negative control mechanism. The nuclear receptors Rev-Erbα and β (NR1D1/2) further regulate the core loop by repressing the transcription of several clock genes, including Bmal1, Clock and Npas2 (Figure 3).
RORs are associated with the circadian clock at several different levels ( Figure 3). First, RORs exhibit a rhythmic pattern of expression in several tissues. In particular, RORγ exhibits a robust oscillatory pattern of expression in liver, brown adipose tissue (BAT), pancreatic β cells, kidney, and small intestines (jejunum), with peak expression around Zeitgeber Time (ZT) 16-18, whereas RORα exhibits no to moderate oscillation in the SCN and several other tissues [94,192,[202][203][204][205]. RORβ2 displays a rhythmic expression pattern in mouse SCN, pineal gland and retina, with a maximum at ZT18 [122,203,206,207]. Several studies showed that RORa and RORc are regulated by Bmal1/Clock and RevErb. This is supported by data indicating that Bmal1, Clock and Rev-Erbα/β were recruited to the E-box and RORE, respectively, in the proximal RORc promoter in mouse liver [192,208,209]. Moreover, Bmal1 and Clock were able to induce activation of the RORc promoter in reporter assays [192,210]. The RORc gene contains two E-box binding sites for Bmal/Clock [192,204,208,211]. Mutation of either E1 or E2 significantly reduced the activation, while the double mutation totally abolished this induction by Clock/Bmal1. The activation of the RORc promoter by Clock/Bmal1 was repressed by Cry1 and correlated with changes in chromatin accessibility at the RORc promoter. Rev-Erbs, rather than Bmal1, regulate the rhythmic expression of RORc [210]. This is supported by data showing that in Bmal1 KO mice, the hepatic expression of RORγ is greatly enhanced particularly at ZT4-8, thereby largely abolishing the robust rhythmic expression pattern of RORγ [210]. The increase in RORγ mRNA expression appeared largely due to the loss of RevErb expression in Bmal1 KO liver, which subsequently abolished the repression of RORc by Rev-Erb at ZT4-8.
A second association between RORs and the circadian clock is their participation in the diurnal regulation of a number of clock genes, including Bmal1, Npas2, Clock, Rev-Erbα, and Cry1 [1,3,24,171,192,202,211,221]. Exogenous expression of RORγ, as well as of RORα, in Hepa1-6 cells enhanced the endogenous expression of Cry1, Bmal1, E4bp4, Clock, Npas2, and Rev-Erbα, whereas treatment with an RORγ antagonist inhibited their induction [24,192,214]. ROREs have been identified in these clock genes [24,192,211,214,218]. Reporter gene and mutation analysis indicated that RORs are involved in the transcriptional regulation of these genes [24,192,[214][215][216][217]. Rev-Erbs, which can compete with RORs for RORE binding, inhibited this activation. ChIP-Seq and ChIP-QPCR analyses further supported the association of RORs with these ROREs in vivo, consistent with the conclusion that these clock genes are directly regulated by RORs. The transcriptional mediator, RIP140, has been shown to be recruited by RORα to the Bmal1 promoter, suggesting that it is involved in mediating the transactivation of Bmal1 by RORα [222].
One might predict that the rhythmic expression of RORs leads to a rhythmic expression of ROR target genes. Indeed, several studies demonstrated that, in addition to clock genes, RORγ also participates in the diurnal regulation of several metabolic genes. Loss of RORγ significantly decreased peak expression of several glucose (e.g., G6pase, Pklr, Glut2, PPARδ) and lipid (e.g., Insig2a, Elovl3, Cyp8b1, Sult1e1) metabolic genes [165,167,168,192]. ChIP analysis showed a ZT-dependent association of RORγ with ROREs in several of these genes. The transcriptional mediator, Prospero-related homeobox 1 (Prox1), which functions as a co-repressor of RORs as well as several other nuclear receptors, was shown to participate in the diurnal regulation of hepatic lipid/glucose metabolism by RORs [223,224]. RORγ-deficient mice exhibited a significantly greater insulin sensitivity and glucose tolerance than WT mice particularly at ZT4-6. Moreover, the conversion of exogenously administered pyruvate to glucose was significantly lower in RORc −/− mice particularly at ZT4-6. Together these findings suggested that RORγ participates in the diurnal regulation of hepatic lipid metabolism, gluconeogenesis and insulin sensitivity. These studies further suggest that RORγ functions as an intermediary between the circadian clock machinery and its regulation of glucose and lipid metabolism.
Recent observations uncovered a connection between RORs and the circadian control of immune functions. RORγt was found to play a role in the diurnal regulation of Th17 differentiation by the circadian clock [225]. In Th17 cells, RORγt is expressed at significantly higher levels at daytime than at nighttime. This diurnal pattern of expression was found to be related to an increase in the daytime expression of Rev-Erb by Bmal1/ Clock, which results in repression of NFIL3 transcription. Since NFIL3 functions as a repressor of RORγt transcription, its repression during daytime alleviates its inhibition of RORγt transcription leading to enhanced RORγt expression. Another study demonstrated that in the ileum, RORα regulates the diurnal expression of several genes associated with TLR signaling [94]. Analysis of gene expression profiles of mucosal biopsies from healthy individuals and patients with inflammatory bowel diseases (IBD) showed that the expression of several circadian genes, including ARNTL2. NPAS2, PER1, and RORA, was upregulated in IBD patients, consistent with a role for these proteins in this pathophysiology [226]. Together, these studies indicate that RORα and RORγ function as a link between the circadian clock and its regulation of various inflammatory pathways and provide a possible mechanism by which disruption of the circadian rhythm is associated with an increased risk of inflammatory diseases.
Clinical studies have indicated an important association between abnormalities in circadian rhythms and patients with mood and neuropsychiatric disorders. Alterations in circadian behavior observed in mice deficient in either RORα or RORβ receptor [122,124,216] and associations between SNPs in RORA and RORB with an increased risk for several neuropsychiatric disorders, including autism spectrum (ASD) and bipolar disorder, schizophrenia, depression, and posttraumatic stress syndrome [106-117, 129, 130], would be consistent with a link between disturbance in the circadian rhythm and these pathologies.

ROR (ant)agonists
There has long been debate about whether RORs function as constitutively active receptors or whether their activity is regulated by (endogenous) ligands that function as an agonist or active antagonist (referred to as inverse agonist) or neutral antagonist [227]. Kallen, Stehlin-Gaon, and co-workers provided the first evidence for the hypothesis that RORs function as ligand-dependent transcription factors [33, 228,229]. Crystal structure analysis revealed that cholesterol and cholesterol sulfate (Figure 4) bind the ligand-binding pocket of RORα and act as RORα agonists [33,228]. Similarly, several retinoids were found to interact with the ligand-binding pocket of RORβ and to function as inverse agonists of RORβ as well as RORγ [229]. Subsequent studies identified a series of oxysterols as ligands for RORα and RORγ [25,32,[230][231][232][233]. For example, 7α-hydroxycholesterol and 24(R)hydroxycholesterol ( Figure 4) were shown to function as inverse agonists, while 25hydroxycholesterol, 20(α)-hydroxycholesterol, 22(R)-hydroxycholesterol, and 7α and 7β 27-hydroxycholesterol act as agonists in mammalian cells. A search for additional ROR ligands led to the discovery of a number of other small molecule modulators of RORγ [25, 32, [234][235][236][237][238][239][240][241][242]. The synthetic LXR agonist T0901317 was found to interact with both RORα and RORγ and to act as an inverse agonist [234]. Through chemical modification of T0901317, Burris and Griffin and coworkers identified a series of related ROR agonists and inverse agonists, such as SR2211 and SR1001 (Figure 4), which do not bind LXR [25, 237,243]. Some of these compounds interacted with both RORα and RORγ, while others were RORα-or RORγ-selective [25, 234,237]. Ursolic acid, a pentacyclic triterpene acid found in many plants, and several vitamin D metabolites, including 20-hydroxyvitamin D, were shown to exhibit RORγ antagonist activity [35,244,245]. Evidence was provided suggesting that these vitamin D metabolites were able to bind the RORγ LBD. A high throughput screen for RORγ ligands led to the identification of the cardiac glycoside, digoxin (Figure 4), and several of its analogs as RORγ antagonists [246]. Subsequently, other investigators set out to discover additional RORγ ligands [32]. This led to the identification of several series of high affinity RORγ inverse agonists, including various sulfonamides, such as GSK3038548A and GNE-3500 (Figure 4)[43, 240-242, 247, 248]. For a comprehensive review of small molecule ligands that interact with and modulate ROR receptors, we refer to several recent reviews [2,25,32,249].
Recently, the connection between sterols and their modulation of ROR activity was further strengthened by studies showing a link between the cholesterol biosynthetic pathway ( Figure  5A) and the regulation of RORγ activity [34,35]. These studies demonstrated that several intermediates of the cholesterol biosynthetic pathway were able to function as endogenous agonists of RORγt. Zymosterol and desmosterol were among the most effective sterols activating RORγ, exhibiting EC50s of 0.11 and 0.08 µM, respectively, while cholesterol exhibited a much lower affinity for RORγ. These sterols enhanced RORγ transcriptional activity as well as the recruitment of coactivators. In addition, these sterols were able to enhance Th17 differentiation and increase the expression of IL-17A [34,35]. Characterization of lipid-bound RORγ complexes immunoprecipitated from mammalian cells supported the concept that cholesterol biosynthetic intermediates function as endogenous RORγ ligands [35]. The connection between RORγ and sterol metabolism was further supported by studies showing that changes in the expression of enzymes involved in the cholesterol biosynthetic pathway were able to modulate RORγ activity. For example, RORγ transcriptional activity was lost in Fdft1-deficient cells lacking squalene synthase, an enzyme acting upstream in the cholesterol biosynthetic pathway [35]. Treatment with azoletype fungicides, such as ketoconazole and clotrimazole, which inhibit the sterol 14αdemethylase cytochrome P450, Cyp51a1, an enzyme upstream in the cholesterol biosynthetic pathway (Figure 5A), caused a dramatic reduction in zymosterol and desmosterol levels and a decrease in RORγ-mediated transactivation, Th17 differentiation, and IL-17 expression [34,250]. Moreover, RORγ-mediated transactivation is greatly diminished in mammalian cells made deficient in Cyp51a1 by shRNA knockdown or germline deletion. Interestingly, several physiological processes that were impaired in RORγ-deficient mice were also affected in Cyp51a1 −/− mice [35]; branchial lymph node anlagen were absent in 75% of Cyp51a1 −/− mice and the number of IL17RA + and CD4 + Lti cells was reduced. In a separate study, mice deficient in the mitochondrial sterol 27hydroxylase (Cyp27A1), a key enzyme in bile acid synthesis and the production of 27hydroxy cholesterol, exhibit a reduction in CD4 + and γδ + T cells and a reduced capacity for Th17 differentiation [233]. These similarities in phenotypic changes are consistent with a link between the cholesterol biosynthetic pathway and RORγ activation. The role of cholesterol synthesis and RORγ activity in Th17 cells was further supported by observations showing that Th17 differentiation is associated with increased cholesterol uptake and biosynthesis and an accumulation of desmosterol that subsequently enhances RORγt activation and Th17 differentiation. In addition, activation of the TCR pathway, which results in activation of SREBP in favor of sterol-sulfate and cholesterol synthesis, might synergize with RORγ in promoting Th17 differentiation and IL-17 synthesis [34,251].
Together, these studies suggest that changes in the cholesterol biosynthetic pathway and the level of cholesterol intermediates by diet or cholesterol-lowering drugs might control RORγ activation and as a consequence influence physiological processes regulated by RORγ, including Th17 differentiation. For example, an increase in Th17 cells and IL-17A under hypercholesterolemic conditions might at least in part be due to an increase in endogenous sterol levels and their subsequent activation of RORγt, while low cholesterol diet might do the inverse. This hypothesis is supported by a study reporting that patients with chronic hepatitis C, which is associated with increased levels of Th17 cells, when placed on a normocaloric, low cholesterol diet showed a significant reduction in Th17 cells and IL-17 levels [252]. Furthermore, treatment with statins, inhibitors of cholesterol synthesis, lead to a reduction in Th17 differentiation and IL-17 production [253].
Several of the sulfated conjugates, such as desmosterol sulfate, have also been shown to activate RORγ at levels twofold higher than the unsulfated sterols. In this context, it is interesting to note that Th17 cell differentiation is accompanied with an increase in the expression of the sulfotransferase, Sult2B1, and reduced expression of the sulfotransferase, STS [34]. This would be consistent with increased synthesis of desmosterol sulfate and RORγ activation and stimulation of Th17 differentiation. Oxysterols exhibit a much lower affinity for RORγ and appear to play a lesser role in modulating RORγ activity in Th17 cells; however, this may depend on the cell type and the type of oxysterol. Interestingly, Cyp7b1 and 3β-hydroxysteroid dehydrogenases (3βHSDs), which are involved in the hydroxylation or dehydrogenation of sterols, have been reported to be regulated by RORα and RORγ [167,185] and therefore might affect the formation of certain (oxy)sterols and as a consequence the activation of RORγ [233].
Many of the ROR (ant)agonists have been shown to bind the ligand-binding pocket within the LBD of RORs [2, 32, 33, 228-232]. As has been reported for other nuclear receptors, agonist binding induces a conformational change in the ROR LBD and realignment of helix 12 that allows release of co-repressor complexes and promotes recruitment of co-activator complexes, which then mediate the transcriptional activation by RORs [1]. Particularly, the PLYKELF sequence within the C-terminal activation function (AF) plays a critical role in ROR transactivation activity and mutations in or deletion of this motif result in a dominantnegative ROR [254,255]. Conversely, binding of ROR antagonists and inverse agonists, such as 25-hydroxycholesterol, inhibits the interaction with co-activators and promote interaction with co-repressors ( Figure 5B). A number of co-repressors and coactivators have been identified that mediate ROR-dependent transcriptional activation, including NCOR, SRC1/2, and RIP140 [36, 222,256].
ROR inverse agonists can inhibit ROR-induced transcriptional activation through different mechanisms. Certain inverse agonists, such as TMP920, have been reported to inhibit RORγ binding to ROREs, whereas the ability of RORγ to bind DNA target sites was mostly preserved with other inverse agonists, such as TMP778 and GSK805 [43]. Interestingly, RORγ cistrome analysis revealed that the latter compounds stabilized RORγt binding to a number of new genomic sites [43]. The distinct effects by various ligands are likely related to the induction of different conformational changes in RORγ that influence its affinity for different ROREs as well as its interaction with other transcriptional mediators. In addition to Th17 related genes, such as Il17 [35,43,244,246], RORγ inverse agonists have been reported to inhibit the expression of a number of RORγ target genes, including the clock genes, Bmal1, Cry1, and Npas2, and several glucose and lipid metabolic genes, such as G6pase, Insig2a,Elovl3,Gck,and PPARδ [24,165,168,192]. RORα inverse agonists and agonists were shown to, respectively, suppress or induce the expression of the RORα target genes, G6pase, Fgf21, CS, and Npas2 [166,187,231].

Summary
The clear evidence that RORγ activity is regulated by endogenous ligands suggests that this is likely the case also for RORα and RORβ. The regulation of RORγ activity by intermediates of the cholesterol biosynthetic pathway suggests that RORγ and the physiological processes controlled by RORγ can be influenced by environmental factors that affect this pathway, including cholesterol-rich or -low diets, environmental agents, such as the azole-type fungicides, and drugs that control cholesterol levels, such as lovastatin. Most importantly, by inhibiting RORγ transcriptional activity and thereby reducing Th17 generation and IL-17A/F production, RORγ inverse agonists may provide a novel strategy in the treatment of various pathologies in which RORγ is implicated, including inflammatory, metabolic, endocrine, and autoimmune diseases [1,2,13,25,257,258]. Similarly, RORα antagonists might affect pathologies by inhibiting the generation of ILC2 cells and other physiological functions and be useful in the management of inflammatory, metabolic, and neuropsychiatric disorders [1,2,13,25,108,[113][114][115][116][117]174]. This concept is supported by reports showing that by inhibiting Th17 differentiation and IL-17 production, RORγ inverse agonists suppress Th17 responses in mice and ameliorate the development of experimental autoimmune encephalomyelitis and imiquimod-induced cutaneous inflammation [43,244,246,259]. The beneficial effects of RORγ antagonists may not only be mediated through the inhibition of IL-17A and IL-17F synthesis in Th17 cells, but also by repressing the synthesis of these and other cytokines in RORγt + innate lymphoid cells (ILC3), and RORγt + γδ T cells, which also play a critical role in several autoimmune and inflammatory diseases [34,77,260,261]. Attenuating RORα/γ activity by antagonist treatment might also be beneficial for the management of metabolic diseases, including metabolic syndrome and insulin resistance [161,165,174,178,190,191]. Recently, the RORα/γ inverse agonist SR1001 was shown to suppress insulitis and prevent hyperglycemia in a mouse model of type 1 diabetes [262]. Together, these studies reinforce the potential of ROR antagonists in the management of autoimmune disease, neuropsychiatric and metabolic disorders, and other pathologies.  In Th0 cells, IRF4 and BATF are bound to chromatin near Th17-associated genes, but the loci are transcriptionally silent. Upon exposure to cytokines, such as IL-6, STAT-3 becomes phosphorylated and transfers to the nucleus, where it binds DNA near IRF4 and BATF and induces Rorc transcription. RORγt can then join the IRF4/BATF/STAT3 transcription factor complex and induce expression of Th17-associated genes, such as Il17 and Il23r. RORs are linked to the circadian clock at different levels: a) ROR expression is regulated by the circadian clock machinery, including Bmal1, Clock, Rev-Erbs and Cry1; b) RORs are involved in the modulation of clock gene expression, including Npas2, Clock and Rev-Erb, and participate in the regulation of the rhythmic expression of glucose and lipid metabolic genes as well as inflammatory genes; c) Deficiency in RORα or RORβ causes changes in the circadian behavior, which might be linked to neuropsychiatric disorders, while deficiency in RORγ leads to increased insulin sensitivity and glucose tolerance and a lower risk of developing diabetes.