Figure 4: Effects of SOF and DAC on CCl4 induced HSCs activation detected by α-SMA. Liver section from control group showed no reaction with α-SMA antibodies (Figure 4A). Treating rats with CCl4 for two weeks resulted in mild reaction with α-SMA stain while six weeks of CCl4 administration resulted in strong positive reaction appeared as brown color in fibrous septa (Figure 4B&C). Figure 4D, E and F demonstrated liver sections from SOF, DAC and combination of both drugs respectively showing milder degree of stain interaction. Figure 4G showed liver section from rats in SOF/DAC group showed nearly negative reaction with α-SMA stain. Dark blue arrows showed the areas of positive reaction with α-SMA antibodies. The original microscopic magnification was 40×.