Table 3: Effect of diclofenac (10mg/kg/i.p.), retigabine (8mg/kg/i.p.), and their combination on hind limb extension duration, frequency of convulsion, frequency of protection, and death rate in the MES test in mice model 3.


Group Parameter Control group (25MA 0. 2S) (N = 6) Diclofenac group (N = 6) Retigabine group (N = 6) Combined group (N = 6) P

HLE (sec) Mean ± SE 16.67 ± 1.78 a 11.75 ± 1.53 b 7 ± 0.75 b no convulsion $ <0.001**
% of change 29.5% 58% α
Frequency of convulsion (%) 100% a 50% b 50% b 0% c # <0.001**
Frequency of protection (%) 0% a 50% b 50% b 100% c
% of change 50% 50% 100%
Death rate (%) 33.3% a 33.3% a 16.7% b 0% b # 0.43 NS
% of change 0 50% 100%

Groups with the same letters are statistically insignificant (p > 0.05).
*significant (p < 0.05). $: ANOVA (F) test. #: Chi-square test. NS: non-significant.
N = the number of animals in each group.
• Mean latency period is the mean period (min) between the injection of picrotoxin and the onset of convulsion.
• HLE hind limb extension duration.