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Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 2 (2012), Issue 2, pp. 13-22
doi:10.11131/2012/101336

Isoprenaline-Induced Myocardial Infarction in Rats: Protective Effects of Hesperidin

Mai A. Zaafan1, Hala F. Zaki2, Amany I. El-Brairy1, and Sanaa A. Kenawy2

1Pharmacology & Toxicology Department, Faculty of Pharmacy, MSA University, Egypt

2Pharmacology & Toxicology Department, Faculty of Pharmacy, Cairo University, Egypt


Copyright © 2012 Mai A. Zaafan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Myocardial infarction is amongst the most common causes of death worldwide. The present study aimed to investigate the cardioprotective effect of hesperidin (200 mg/kg) either individually or in combination with atorvastatin (10 mg/kg), as a reference standard, in isoprenaline-induced myocardial infarction in rats. Markers chosen to assess cardiac damage included serum activity of creatine kinase-MB (CK-MB) and serum level of cardiac troponin-I (cTn-I), oxidative stress biomarkers including cardiac contents of malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO) as well as serum levels of C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10). Furthermore, ECG monitoring and histologic examinations of cardiac tissues were done. Isoprenaline increased CK-MB activity as the well the levels of cTn-I, inflammatory and oxidative stress biomarkers. In addition, it produced ST segment elevation and degenerative changes in heart tissues. The obtained data revealed that pretreatment with hesperidin alone or in combination with atorvastatin significantly decreased the elevated activity of serum CK-MB as well as serum levels of cTn-I, CRP, TNF-α and IL-10 coupled by a reduction in cardiac lipid peroxides and NO content. Moreover, both treatments resulted in marked improvement in isoprenaline-induced ECG and histopathologic changes. In conclusion, hesperidin can be regarded as a promising cardioprotective natural agent in myocardial infarction when used alone or combined with atorvastatin.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 2 (2012), Issue 2, pp. 13-22
doi:10.11131/2012/101336

Isoprenaline-Induced Myocardial Infarction in Rats: Protective Effects of Hesperidin

Mai A. Zaafan1, Hala F. Zaki2, Amany I. El-Brairy1, and Sanaa A. Kenawy2

1Pharmacology & Toxicology Department, Faculty of Pharmacy, MSA University, Egypt

2Pharmacology & Toxicology Department, Faculty of Pharmacy, Cairo University, Egypt


Copyright © 2012 Mai A. Zaafan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Myocardial infarction is amongst the most common causes of death worldwide. The present study aimed to investigate the cardioprotective effect of hesperidin (200 mg/kg) either individually or in combination with atorvastatin (10 mg/kg), as a reference standard, in isoprenaline-induced myocardial infarction in rats. Markers chosen to assess cardiac damage included serum activity of creatine kinase-MB (CK-MB) and serum level of cardiac troponin-I (cTn-I), oxidative stress biomarkers including cardiac contents of malondialdehyde (MDA), reduced glutathione (GSH) and nitric oxide (NO) as well as serum levels of C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α) and interleukin-10 (IL-10). Furthermore, ECG monitoring and histologic examinations of cardiac tissues were done. Isoprenaline increased CK-MB activity as the well the levels of cTn-I, inflammatory and oxidative stress biomarkers. In addition, it produced ST segment elevation and degenerative changes in heart tissues. The obtained data revealed that pretreatment with hesperidin alone or in combination with atorvastatin significantly decreased the elevated activity of serum CK-MB as well as serum levels of cTn-I, CRP, TNF-α and IL-10 coupled by a reduction in cardiac lipid peroxides and NO content. Moreover, both treatments resulted in marked improvement in isoprenaline-induced ECG and histopathologic changes. In conclusion, hesperidin can be regarded as a promising cardioprotective natural agent in myocardial infarction when used alone or combined with atorvastatin.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 2 (2012), Issue 2, pp. 13-22
doi:10.11131/2012/101336

Isoprenaline-Induced Myocardial Infarction in Rats: Protective Effects of Hesperidin

Mai A. Zaafan1, Hala F. Zaki2, Amany I. El-Brairy1, and Sanaa A. Kenawy2

1Pharmacology & Toxicology Department, Faculty of Pharmacy, MSA University, Egypt

2Pharmacology & Toxicology Department, Faculty of Pharmacy, Cairo University, Egypt


Copyright © 2012 Mai A. Zaafan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite this Article

Mai A. Zaafan, Hala F. Zaki, Amany I. El-Brairy, and Sanaa A. Kenawy, “Isoprenaline-Induced Myocardial Infarction in Rats: Protective Effects of Hesperidin,” Egyptian Journal of Basic and Clinical Pharmacology, Vol. 2, Issue 2, pp. 13-22, 2012. doi:10.11131/2012/101336