• 304 Views
  • 108 Downloads
Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 1 (2011), Issue 1, pp. 43-50
doi:10.11131/2011/101330

Effect of Long-Term Treatment with Fluoxetine, Clomipramine and St. John's Wort Extract on Bone Turnover in Female Irradiated Rats

Mona A. Elghazaly1, Walaa A. Elsabbagh1, Hala F. Zaki2, Laila A. Ramadan1, and Sanaa A. Kenawy2

1Drug Radiation Research Department, National Center for Radiation Research and Technology, Cairo, Egypt

2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt


Copyright © 2011 Mona A. Elghazaly et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim: The study aimed at evaluating the bone turnover rate in irradiated female rats treated daily with fluoxetine, clomipramine or St. John’s wort extract for 8 weeks. Material and Methods: 40 rats were randomly classified into 5 experimental groups: normal, irradiated control, irradiated fluoxetine, irradiated clomipramine and irradiated St. John’s wort extract treated groups. The irradiated animals were exposed to a total dose of 15 Gy, fractionated over 5 weeks to small doses each of 1 Gy (day after day). Bone turnover rate biomarkers [serum osteocalcin (OC), urinary hydroxyproline/ creatinine ratio (Hpr/ Cr), urinary calcium/creatinine ratio (Ca/Cr)], hypothalamic pituitary adrenal [serum corticosterone], thyroid activities [serum thyroxin (T4) and thyrotrophin (TSH)], antioxidant [serum total antioxidant capacity (TAC) and malondialdehyde (MDA)] and pro-inflammatory biomarkers [serum tumor necrosis factor-alpha (TNF-α)] were done after 8 weeks from the 1st exposure to radiation. Histopathological investigations were also performed. Results: The present results revealed that irradiation induced a significant decrease in serum OC by 43.6%, and a significant increase of Hpr/ Cr and Ca/Cr by 186.4% and 192.4%, respectively. Irradiated rats showed also a significant increase in serum corticosterone, TNF-α and MDA as well as a significant decrease in serum T4, TSH levels and TAC. Treatment of irradiated rats with St. John’s wort extract, fluoxetine or clomipramine ameliorated most of the changes caused by bone-irradiation. The latter findings were confirmed by histological examination of bone tissue. Conclusions: St. John’s wort extract offers a therapeutic potential on bone comparable to traditional antidepressants like fluoxetine or clomipramine in irradiated female rats.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 1 (2011), Issue 1, pp. 43-50
doi:10.11131/2011/101330

Effect of Long-Term Treatment with Fluoxetine, Clomipramine and St. John's Wort Extract on Bone Turnover in Female Irradiated Rats

Mona A. Elghazaly1, Walaa A. Elsabbagh1, Hala F. Zaki2, Laila A. Ramadan1, and Sanaa A. Kenawy2

1Drug Radiation Research Department, National Center for Radiation Research and Technology, Cairo, Egypt

2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt


Copyright © 2011 Mona A. Elghazaly et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim: The study aimed at evaluating the bone turnover rate in irradiated female rats treated daily with fluoxetine, clomipramine or St. John’s wort extract for 8 weeks. Material and Methods: 40 rats were randomly classified into 5 experimental groups: normal, irradiated control, irradiated fluoxetine, irradiated clomipramine and irradiated St. John’s wort extract treated groups. The irradiated animals were exposed to a total dose of 15 Gy, fractionated over 5 weeks to small doses each of 1 Gy (day after day). Bone turnover rate biomarkers [serum osteocalcin (OC), urinary hydroxyproline/ creatinine ratio (Hpr/ Cr), urinary calcium/creatinine ratio (Ca/Cr)], hypothalamic pituitary adrenal [serum corticosterone], thyroid activities [serum thyroxin (T4) and thyrotrophin (TSH)], antioxidant [serum total antioxidant capacity (TAC) and malondialdehyde (MDA)] and pro-inflammatory biomarkers [serum tumor necrosis factor-alpha (TNF-α)] were done after 8 weeks from the 1st exposure to radiation. Histopathological investigations were also performed. Results: The present results revealed that irradiation induced a significant decrease in serum OC by 43.6%, and a significant increase of Hpr/ Cr and Ca/Cr by 186.4% and 192.4%, respectively. Irradiated rats showed also a significant increase in serum corticosterone, TNF-α and MDA as well as a significant decrease in serum T4, TSH levels and TAC. Treatment of irradiated rats with St. John’s wort extract, fluoxetine or clomipramine ameliorated most of the changes caused by bone-irradiation. The latter findings were confirmed by histological examination of bone tissue. Conclusions: St. John’s wort extract offers a therapeutic potential on bone comparable to traditional antidepressants like fluoxetine or clomipramine in irradiated female rats.

Original Article
Egyptian Journal of Basic and Clinical Pharmacology
Vol. 1 (2011), Issue 1, pp. 43-50
doi:10.11131/2011/101330

Effect of Long-Term Treatment with Fluoxetine, Clomipramine and St. John's Wort Extract on Bone Turnover in Female Irradiated Rats

Mona A. Elghazaly1, Walaa A. Elsabbagh1, Hala F. Zaki2, Laila A. Ramadan1, and Sanaa A. Kenawy2

1Drug Radiation Research Department, National Center for Radiation Research and Technology, Cairo, Egypt

2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Cairo, Egypt


Copyright © 2011 Mona A. Elghazaly et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

How to Cite this Article

Mona A. Elghazaly, Walaa A. Elsabbagh, Hala F. Zaki, Laila A. Ramadan, and Sanaa A. Kenawy, “Effect of Long-Term Treatment with Fluoxetine, Clomipramine and St. John's Wort Extract on Bone Turnover in Female Irradiated Rats,” Egyptian Journal of Basic and Clinical Pharmacology, vol. 1, Issue 1, pp. 43-50, 2011. doi:10.11131/2011/101330